This comprehensive text gives a practical introduction to the techniques of neural transplantation in mammalian brain tissue. Written by leading experts in the field, it provides a detailed description of the techniques and offers useful details and advice complementing the underlying principles. Each chapter identifies the equipment needed, the preparations to be made in advance, and step-by-step protocols for each procedure. All aspects of neural transplantation surgery are covered, from staging and dissection of embryonic CNS tissues, through the preparation of tissues for implantation, to the transplant surgery itself. A variety of methods for solid and cell suspension transplantation are discussed. Specialized chapters consider genetic engineering of cells, labeling of grafts, and other aspects of tissue manipulation of PNS and gilal tissues; transplantation in neonatal animals, including primates; handling of human embryonic tissues for transplantation; and immunological considerations. This is the only volume dedicated to technical, procedural and laboratory manual accounts, making it indispensable to neuroscientists, neurologists, and neurosurgeons interested in this growing area of research.
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